Journal: Poultry Science

Article Title: New insights into the spleen injury by mitochondrial dysfunction of chicken under polystyrene microplastics stress

doi: 10.1016/j.psj.2024.103674

Figure Lengend Snippet: The primers involved in this study.

Article Snippet: GPX4 , 1:500 , Boster Biotechnology, China , A02059-1.

Techniques:

Journal: Poultry Science

Article Title: New insights into the spleen injury by mitochondrial dysfunction of chicken under polystyrene microplastics stress

doi: 10.1016/j.psj.2024.103674

Figure Lengend Snippet: The antibodies used in this study.

Article Snippet: GPX4 , 1:500 , Boster Biotechnology, China , A02059-1.

Techniques:

Journal: Poultry Science

Article Title: New insights into the spleen injury by mitochondrial dysfunction of chicken under polystyrene microplastics stress

doi: 10.1016/j.psj.2024.103674

Figure Lengend Snippet: MPs induced ferroptosis in spleen. (A) Western Blot results in ferroptosis-related proteins. (B) Quantitative analysis of GPX4, FTH1, and SLC7A11 protein expression. (C) Ferroptosis-related factors of mRNA expression changes. Data were expressed as mean ± SD, n = 3. *, **, ***, denotes: p < 0.05, 0.01, and 0.001, respectively.

Article Snippet: GPX4 , 1:500 , Boster Biotechnology, China , A02059-1.

Techniques: Western Blot, Expressing

Journal: Journal of Virology

Article Title: Chikungunya Virus Vaccine Candidates with Decreased Mutational Robustness Are Attenuated In Vivo and Have Compromised Transmissibility

doi: 10.1128/JVI.00775-19

Figure Lengend Snippet: Stop and SuperStop protect against challenge infection. Sera from mice infected with WT, Stop, or SS virus or mock infected with PBS (WT-s, Stop-s, SS-s, or PBS-s) were transferred to C57BL/6 mice that had previously received type I IFN-α/β receptor antibody and were challenged with WT CHIKV. (A) Survival after challenge of mice ( n = 5). *, P = 0.0116 (Kaplan-Meyer with Mantel-Cox test). (B) Weight change after challenge. Values are means with standard errors of the means ( n = 5). Day 4 WT-s *, P = 0.0174; day 4 Stop-s *, P = 0.0218; day 4 SS-s **, P = 0.098; day 5 WT-s **, P = 0.0068; day 5 SS-s *, P = 0.0198; day 6, WT-s **, P = 0.0016; day 6 SS-s *, P = 0.0187 (two-way analysis of variance with Tukey’s multiple-comparison test). (C) Viremia was measured at days 1, 3, 4, 5, 7, and 9 postchallenge. Values are means with standard errors of the means ( n = 5). For days 1, 3, 4, 5 and 7, values for WT-s, Stop-s, and SS-s were significantly different from the value for PBS-s (****, P < 0.0001) (two-way ANOVA with Tukey’s multiple-comparison test). (D) Footpad swelling at days 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 17, 21, 25, and 34 postchallenge. Values are means with standard errors of the means ( n = 5). Values for WT-s, Stop-s, and SS-s were significantly different from the value for PBS-s at days 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 17, and 21 (****, P < 0.0001) and at day 25 (*, P < 0.05) (two-way analysis of variance with Tukey’s multiple-comparison test). (E) Neutralizing antibody titers for individual serum samples and pooled (p) sera (used in passive transfer) from mice infected with WT (WT-p), Stop (Stop-p), and SS (SS-p) virus and mock infected with PBS (PBS-p). Values are means with standard errors of the means ( n = 5). Differences in the values for Stop-s ( P = 0.3210) and SS-s ( P = 0.1335) were nonsignificant ( P > 0.05) relative to values for WT-s. ****, P < 0.0001 (one-way analysis of variance with Tukey’s multiple-comparison test). For pooled sera ( n = 1), no statistical tests were performed.

Article Snippet: In parallel, 0.1 mg of type I IFN-α/β receptor antibody (BD Biosciences) was administered to mice by i.p. injection to increase their susceptibility to CHIKV infection.

Techniques: Infection